SYLLABUS AND EXAMINATION PLANING FOR BIOCHEMIST

RAJASTHAN PUBLIC SERVICE COMMISSION, AJMER 

SYLLABUS AND EXAMINATION PLANNING FOR THE POST OF BIOCHEMIST

(MEDICAL EDUCATION DEPARTMENT) 

EXAMINATION PATTERN:

Pattern of Question Papers: 

1. Objective Type Paper 

2. Maximum Marks : 100 

3. Number of Questions : 100 

4. Duration of Paper : Two Hours 

5. All Questions carry equal marks 

6. There will be Negative Marking.

 

:SYLLABUS OF EXAMINATION:

1. Basic Laboratory Procedures: 

• Disinfection, Laboratory safety, first aid. 
• Biochemical samples and their collection, preservatives, anticoagulants. 
• Preparation of solutions and reagents. 
• Laboratory reporting systems, biological variations, chemical interference, Normal/reference range.
• Expression of results of biochemical investigations various units. 
• Quality management of laboratory – standardization and calibration. 
• Biomedical waste disposal. 
• Laboratory record keeping, Accreditation. 

2. Biochemical Instruments/Technique: Colorimetry, Spectrophotometry, Fluorometry, Chromatography, Electrophoresis, Densitometer, Flame photometry, Electrolyte analyser, Blood gas analyser, Atomic absorption spectroscopy, RIA, ELISA, Gamma counter, Chemiluminescence assay, Water purification systems, Semi auto analyser, Fully autoanalyzer, PCR, HPLC, Blotting technique. 

3. Estimation of various biochemical parameters. 

4. Organ function tests: Liver, kidney, thyroid, pancreas, adrenocortical. 

5. Chemistry, metabolism & Inborn errors of: 

1.  Carbohydrates 
2. Lipids 
3.  Amino Acid & Proteins 
4. Nucleotides 

6. Enzymes 

7. Vitamins 8. Mineral Metabolism and abnormalities 

9. Acid-base balance & pH 

10. Electrolyte and water balance. 

11. Heme synthesis & Breakdown 

12. Energy metabolism & Nutrition 

13. Hormones 

14. Environmental Biochemistry 

15. Immunochemistry 

16. Biochemistry of Cancer 

17. Biochemistry of HIV & AIDS